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Synopsis Rbhu (cell) is the first, who get synthesized in the water at its own, gradually they get developed gladdening drop (Life energy), genetic material and self nourshment capacity, thought development of protochlorophy II from its outer wall, the cell became well-equippd. It seems that the fundamental energy is the original source of the creation, from where the different components of the nature have been synthesized through energy transformation under temperature pressure gradient. The first cell was get synthesized in the water at its own under natural conditions, with energy transformation in side a closed system, it has acquired a life energy, this energy can be generated due to the movements of the charged particles in side a closed system is the first symbol of life. It has maintained its continuity through cell division, with the evolution of living beings in the nature, the life energy is also having its evolution, which gradually attains stability in young cells with metabolic reactions and under go gradually towards its end through ageing of the cells. Among Living beings, human species is highly evolved, which shows evolution of mental qualities under the cycle of rebirth. According to the principles of evolution, Evolution may be progressive or retrogressive, accordingly Bio Soul may lead it to retrogressive evolution, according to the thinking and attitude of a individual in the present life as Bio Soul (DNA) is immortal in the sense that it maintains its continuity through its offsprings.