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Synopsis Man is considered to be the most powerful creature in universe, but he depends totally on the biological entities for his all nutritional needs. Biotechnology use biological entitles for the betterment of human kind. Biotechnology derived from fusion of biology and technology, is a very vast subject which includes Plant cell and tissue culture, Animal cell and Tissue culture, Transgenic, Molecular biology, recombinant DNA technology, genetic engineering, Medical biotechnology, Fermentation technology, Microbiology, Enzyme technology, Biofuels, Environmental biotechnology, Bioniformatics and Nanotechnology. It may be pointed out that use of biological entitles is not new. Ever since establishment of civilization man is using biological organisms to satisfy his needs. But this can not be regarded as biotechnology pertaining to agriculture and animal husbandry etc. has now become distinct disciplines. However exploration of plant and animal cell in vitro as well as their constituents to generate some useful product constitute biotechnology since production technology pertaining to agriculture and animal husbandry etc. has now become distinct disciplines. However exploration of plant and animal cell in vitra as well as their constituents to generate some useful product constitute biotechnology. Animal cell and tissue culture is an integral part of Biotechnology and this book covers all the aspects of animal cell culture. Animal cells are used for making new vaccines, specific animal proteins such as intergerons, blood factors and hormones, monoclonal antibodies for use as diagnostic and therapeutics, gene probes as diagnostic too, enzymes and last but not the least many new and important compounds. This book is beneficial to all students of under graduation as well as post graduation of all universities. This book comprises 13 chapters covering all aspects of Animal cell Biotechnology and very recent area of research i.e. Tissue engineering has also been included with updated text materials and graphics. Chapter 1 of this book describes the general introduction and basics about animal cell and tissue culture. Chapter 2, 3 and 4 describes how to get start with animal cell culture and to establish cell lines for further studies use? Chapter 5 and 6 tells about the selection and preservation of animal cells which becomes necessary if we have to deal with genetically modified/clones cells. Chapter 8 covers all aspects of fermentors used in scale up progress of animal cells. Chapter 9 explains the most extensive use of animal cell culture ie.e. hybridoma technology and production of monoclonal antibodies. Genetic manipulation of animal cells (trasgenesis), a relatively new field is dealt in chapter 10 and its applications are given in chapter 11 which are very useful to all students of biotechnology as well as other students of relevant subjects. Chapter 12 is the most important, which enhances the importance of this book. Tissue engineering is an emerging area of research in medical biotechnology and has shown good future for transplantation biology. Similarly chapter 13 is comprising new field of biotechnology; the Gene therapy. Gene Therapy has already proven its importance diseases like SCID, cystic fibrosis etc.