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In the face of growing demand for more food crops, agricultural yield is challenged by two concerns: availability of arable land and reduced water resources for irrigation. Biotechnology offers several tools that can be used appropriately for sustainable agriculture. Recent advances in molecular biology and recombinant DNA Technology can make improved production and pest resistant crops with increased nutritive value a reality.The book has 21 chapters contributed by eminent scientists from all over the country. It discusses the various techniques and aspects of plant tissue culture and gene transfer that can bring about crop improvement. The topics discussed include: Hormonal regulation; Micropropagation; In vitro pollination and fertilization; Embryo culture. Protoplast isolation and culture; Somatic hybridization; Synthetic seeds. Methods of genetic transformation in plants; Germplasm storage; GM technology, biosafety regulations and patents; Molecular markers; Conservation of medicinal and aromatic plants; The book will be useful for all undergraduates, postgraduates and researchers studying plant biotechnology.