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In recent years biotechnology has created a new vistas in the field of science and technology. The application of biotechnology in the field of environmental sciences has remained neglected till recently. First biotechnology is not a discipline but a field of activity in the environmental conservation, protection and above all application to ameliorate the dwindling scenario of natural resources. Secondly, genetic engineering per se is not a discipline but an exciting development which will have an enormous impact on biotechnology. We have tried to draw attention to possible scientific technological and also raise a number of environmental issues for further debate which are linked, directly or indirectly, with policies on environment. A short glossary of commonly used scientific terms have also been provided. Here is this book, a manual for plant cell and tissue culture, a tool for both environment and biotechnology.